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Abstract

To determine the percent of occurrence of Mycoplasma spp. in sterile pyuria and the evaluation of e-Myco PCR as rapid and sensitive technique for identification. Sterile pyuria urine samples collected from 55 patients attending to Al Diwania Teaching Hospital and different private laboratories in Al-Diwania province were tested by polymerase chain reaction (PCR) for the presence of Mycoplasma spp. by a new kit called e-Myco in addition to 20 healthy individual as control group. In the present study the ages of patients with sterile pyuria were (20-57) year old and the age group (20-30 years) recorded the highest incidence of sterile pyuria 31/55 (56.37%). From total 55 sterile pyuria patients 21 (38%) patients were positive for Mycoplasma spp. by direct detection by PCR with significant difference (P<0.05). On the other hand 7/21( 12.7% ) patients were with recurrent urinary tract infection, 4/11 (7.2%) patients with primary and secondary infertility, in 3/3 (5.5%) patients with urinary bladder cancer, in 3/6 (5.5%) patients with chronic prostitis and 4/14 (7.2%) in non complications patients were positive for Mycoplasma spp. PCR assay provide a rapid and effective measure to detect Mycoplasma spp. in sterile pyuria. The incidence of sterile pyuria decrease with progress in age.

Article Type

Article

First Page

144

Last Page

153

Rights

Copyright (c) 2017 AL-QADISIYAH MEDICAL JOURNAL

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