Abstract
Four hindered seventy six clinical samples were collected from patients since June 2003 to May 2004 the number of Staphylococcus aureus isolated from the samples were 163 isolates as follows : urine 52 isolates , wound swab 38 isolates , ear swab 21 isolates , tonsillar swab 17 isolates. Boil swab 11 isolates and Nasal swab 24 isolates Alpha hemolysin of Staphylococcus aureus isolates was designated by hemolysin assay it was found that 42% ( 70 ) of isolates were alpha- hemolysin producers. The extraction of alpha- hemolysin from liquid media from producing isolates was done by cooling centrifugation and purified by ammonium sulphate precipitation and gel - filtration chromatography. Activities of purified alpha-hemolysin were detected, it had 2560 HU/ml the optimum temperature of alpha - hemolysin activity was 20 - 40 C and the optimum incubation period was 14hr. The optimum pH of alpha-hemolysin activity was 7. Cytotoxic activity of alpha - hemolysin against human leukocytes were determined and found the survival ratio of human leukocytes was decreased (38%) after treatment with 15 mg/ml for 30 min. while it lost after treatment with 30 mg/ml for 30 min. too.
Article Type
Article
Recommended Citation
Hassen, Talib Falih
(2026)
"Extraction and purification of alpha- hemolysin which producing by Staphylococcus aureus isolated from different Clinical samples,"
Al-Qadisiyah Medical Journal: Vol. 4:
Iss.
5, Article 9.
DOI: 10.28922/qmj.2008.4.5.69-80
Available at:
https://qmj.researchcommons.org/journal/vol4/iss5/9
First Page
69
Last Page
80
Rights
Copyright (c) 2017 AL-QADISIYAH MEDICAL JOURNAL
