Detection of Extended Spectrum β-lactamases Producing by Klebsiella pneumonia Isolated from Urinary tract Infection Patients by Using mPCR Technique

Authors

Wafaa Abdelwahed Jaheel Al-Kaaby, College of Biotechnology/ Al-Qadisiya University

Abstract

Objective: In this study Klebsiella pneumonia where isolated from urine samples and detection of extended spectrum β-Lactamases by using mPCR technique.Method: This isolated subjected to the antimicrobial susceptibility test (Disc diffusion) for some antibiotics following by multiplex PCR techniques for detection extended spectrum β-Lactamase genes (bla TEM, bla SHV, bla CTX-M and bla AMPC).Results: The extended spectrum β-Lactamase genes by PCR techniques were given bla CTX-M (30/93.75%), bla SHV (25/78.12%), bla TEM (18/56.25%) and bla AMPC (22/68.15%), respectively.Conclusion: K. pneumonia isolates of urinary tract infection patients highly associated with the emergence of bla CTX-M β-Lactamase that provides useful good treatment.

Article Type

Article

Recommended Citation

Jaheel Al-Kaaby, Wafaa Abdelwahed (2026) "Detection of Extended Spectrum β-lactamases Producing by Klebsiella pneumonia Isolated from Urinary tract Infection Patients by Using mPCR Technique," Al-Qadisiyah Medical Journal: Vol. 12: Iss. 22, Article 8.
DOI: 10.28922/qmj.2016.12.22.59-64
Available at: https://qmj.researchcommons.org/journal/vol12/iss22/8

First Page

59

Last Page

64

Rights

Copyright (c) 2017 AL-QADISIYAH MEDICAL JOURNAL